If I get up at 3 AM to urinate, should I test then?

Testing the urine pH after 6 hours or more of sleep is ideal. Not everyone can do this, however. If you wake at night to urinate, simply measure your pH first thing in the morning when you get up to stay. Also, if you go to bed at 10 and wake up at 4, you can measure your urine then at 4 after 6 hours of sleep, but this means turning on the light which might disrupt getting back to sleep.